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Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Special Issue 5 (2019)

Effect of colchicine treatment on survival of seedlings and morphology in Cape gooseberry (<em>Physalis peruviana</em> L.)

Author(s):

Ravindra Kumar, KK Jha, S Sengupta, S Misra, CS Mahto and SC Narayan

Abstract:
Polyploidy plays an active role in evolution of plants. Thus, induction of polyploidy particularly at autotetraploid and triploid levels offers probably the best scope for seedless as well as quality fruits. So, this method is one of the best options for the chromosome manipulation. In the present study an attempt was made to induce polyploids in Cape gooseberry using colchicine with the objective of creating more genetic variability. The colchicine concentrations used were 0.10(C<sub>1</sub>), 0.20(C<sub>2</sub>) and 0.40(C<sub>3</sub>) per cent for the duration 12 (H<sub>1</sub>), 24 (H<sub>2</sub>) and 36 (H<sub>3</sub>) hours with seedling apex dip method (M<sub>1</sub>), cotton plug method (M<sub>2</sub>) and lanolin paste method (M<sub>3</sub>). The growing apex of seedlings were treated with cotton swab C<sub>1 </sub>(0.10), C<sub>2 </sub>(0.20) and C<sub>3 </sub>(0.40) per cent aqueous solution of colchicine for 12 (H<sub>1</sub>), 24 (H<sub>2</sub>) and 36(H<sub>3</sub>) hours resulted into seedlings survival, maximum 87.50 per cent in 2017-18 and 89.58 per cent in 2018-19 with treatment C<sub>1</sub>M<sub>2</sub>H<sub>1</sub> (0.10% for 12 hours with cotton plug method) whereas expected ploidy levels 10.42 per cent and 12.50 per cent respectively in both the years with same treatment. On the basis of findings, seedling survival was less in apex dip method in comparison to the cotton plug method and lanolin paste method. During the morphological observation the results indicated that 0.10 per cent (C<sub>1</sub>) of colchicine had more capacity to induce the polyploidy in seedlings rather than apex dip method (M<sub>1</sub>) or cotton plug method (M<sub>2</sub>).

Pages: 184-189  |  1415 Views  483 Downloads

How to cite this article:
Ravindra Kumar, KK Jha, S Sengupta, S Misra, CS Mahto and SC Narayan. Effect of colchicine treatment on survival of seedlings and morphology in Cape gooseberry (<em>Physalis peruviana</em> L.). J Pharmacogn Phytochem 2019;8(5S):184-189.

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