Author(s):

Janak Raj Bohara, Sarju Maharjan, Asmita Poudel, Kasmita Karki, Vivek Bist, Rajendra Regmi, Santosh Marahatta and Lekhnath Kafle

Abstract: An experiment was conducted to evaluate the effect of different concentration of entomopathogenic fungus, Metarhizium anisopliae (Metsch.) Sorokin against white grubs in the Entomology Laboratory, at Institute of Agriculture and Animal Science, Rampur, Chitwan in 2015. For the experiment, 6 treatments were tested against white grubs at the rate of 10⁷ commercial conidial spores per ml and indigenous 10⁷, 10⁵, 10⁴, 10² of conidial spores per ml including control and each treatment were replicated thrice. Total 270 grubs were treated, with 15 grubs in each replication of each treatment. They were screened for relative pathogenicity, and the mean lethal time eliciting 50% mortality (LT₅₀) was calculated. Indigenous 10⁴ spores/ml concentration of fungus had the lowest LT₅₀ value of 61.45 days followed by 10² spores/ml with 65.35, 10⁷ spores/ml with 68.98, 10⁵ spores/ml with 76.04 and commercial 10⁷ spores/ml with 152.71 days. Indigenous isolate showed higher mortality of white grubs in comparison to commercial isolate

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