Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp.

Results: Preliminary phytochemical screening of petroleum ether extract of Holoptelea integrifolia showed the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins and carbohydrates while methanolic extract of Holoptelea integrifolia showed the presence of steroids, alkaloids, flavonoids, proteins and carbohydrates. HPTLC finger printing of alkaloids of petroleum ether extract of leaves revealed eight polyvalent phytoconstituents (8 peaks) and corresponding ascending order of R_f values in the range of 0.05 to 0.72. while methanol extract of leaves showed six polyvalent phytoconstituents (6peaks) and corresponding ascending order of R_f values in the range of 0.11 to 0.67.

Conclusions: With the results of preliminary phytochemical analysis and above R_f values we have concluded the presence of alkaloids in both the extracts.