• Printed Journal
  • Indexed Journal
  • Refereed Journal
  • Peer Reviewed Journal
Journal of Pharmacognosy and Phytochemistry

Journal of Pharmacognosy and Phytochemistry

Vol. 8, Issue 3 (2019)

Isolation and characterization of urolithin b from asphaltum

Author(s):

Shefali Thakkar and Kirti Laddha

Abstract:
Asphaltum (vernacular name: Shilajit), is a traditional Ayurvedic medicine used as aphrodisiac. The present study was aimed for extraction, isolation, method development and validation of RP-HPLC method for bioactive compound called urolithin B (benzocoumarin) for Asphaltum. HPLC method was developed with the help of PDA detector for isolated compound. Method validation was performed as per International Conference of Harmonization guidelines for linearity, accuracy, precision, specificity, limit of detection and limit of quantification of urolithin B. Intra-day and Inter-day precisions for urolithin B was found precise as ≤ 2.0% in intraday exercise & ≤ 3.0% in inter day exercise. The concentration found in Asphaltum is 0.38% of urolithin B. The proposed method for estimation of urolithin B is found to be accurate, precise, reproducible and repeatable.

Pages: 138-140  |  1941 Views  529 Downloads


Journal of Pharmacognosy and Phytochemistry Journal of Pharmacognosy and Phytochemistry
How to cite this article:
Shefali Thakkar and Kirti Laddha. Isolation and characterization of urolithin b from asphaltum. J Pharmacogn Phytochem 2019;8(3):138-140. DOI: 10.22271/phyto.2019.v8.i3c.8066

Call for book chapter